Moxibustion relieves heart disease

Moxibustion is a traditional Chinese medicine preparation, which has been clinically used to treat cardiac diseases in recent years. The present study aimed to examine the protective effects and possible mechanisms of moxibustion on doxorubicin-induced chronic heart failure in rats.

The animals were divided into five groups, including the Control (normal saline), doxorubicin (doxorubicin 15 mg/kg), moxibustion (doxorubicin 15 mg/kg + moxibustion), BEN (doxorubicin 15 mg/kg + benazepril 0.86 mg/kg) and moxibustion + BEN (doxorubicin 15 mg/kg + moxibustion + benazepril 0.86 mg/kg) groups. After three weeks, echocardiography was performed to assess cardiac function and structure, including left ventricular internal diameter in systole, ejection fraction and fractional shortening (FS).

Serum brain natriuretic peptide levels and adenosine triphosphate (ATP) levels were measured by enzyme-linked immunosorbent assay and ATP assay. Cardiac pathology was assessed by hematoxylin and eosin and Masson’s trichrome staining. Cardiac ultrastructure and the number of autophagosomes formed were visualized by transmission electron microscopy. Western blotting was performed to assess mitochondrial dynamics, autophagy proteins and mitochondrial autophagy-related pathway proteins. The expression levels of these genes were assessed by reverse transcription-quantitative PCR.

The results indicated moxibustion could improve cardiac function, increased cardiac ATP levels and reduced myocardial fibrosis. Western blotting indicated that moxibustion treatment elevated the expression of optic atrophy 1 protein (OPA1), while decreasing the expression of dynamin-related protein 1 and mitochondrial fission 1 protein. In addition, moxibustion inhibited autophagy, as evidenced by decreased number of autophagosomes, reduced LC3II/LC3I ratio and increased p62 expression. Furthermore, moxibustion downregulated doxorubicin-induced FUNDC1 signaling pathway.

In summary, moxibustion has protective effects on doxorubicin-induced chronic heart failure in rats, promoting mitochondrial fusion while inhibiting mitochondrial fission and mitophagy. The underlying mechanisms may be related to the inhibition of the FUNDC1 signaling pathway.


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About Attilio

Doctor of Chinese medicine, acupuncture expert and author of My Fertility Guide and My Pregnancy Guide.

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