Background: This study aims to analyze the mechanism of Gua Lou Xie Bai Gui Zhi Tang in treating coronary heart disease utilizing network pharmacology.
Methods: The Gua Lou Xie Bai Gui Zhi Tang effective components were searched on the pharmacological database platform of the Traditional Chinese Medicine Systems Pharmacol, and its potential target was predicted. The Online Mendelian Inheritance obtained coronary heart disease disease target in Man and GeneCards database. The Venn map of the intersection target for Gua Lou Xie Bai Gui Zhi Tang and coronary heart disease was constructed by using Venn online website.
The “drug-component-target-disease” network map was constructed by Cytoscape 3.7.2 software. The DAVID online platform was used to analyze the function of Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) at the intersection of targets of drugs and diseases.
Results: A total of 27 articles were searched for Gua Lou Xie Bai Gui Zhi Tang, including 111 potential targets, 5521 disease targets, 100 drug and disease intersection targets. The core target network map shows that Interleukin (IL)-6, TNF, vascular endothelial growth factor (VEGFA), TP53, EGF, JUN, MAPK1, Catalase (CAT), and prostaglandin-endoperoxide synthase 2 (PTGS2) may be the key targets in coronary heart disease therapy. Gene Ontology functional enrichment analysis revealed that the biological functions of Gua Lou Xie Bai Gui Zhi Tang involved biological processes such as response to drugs, positive regulation of nitric oxide biosynthesis process, and response to hypoxia.
KEGG pathway enrichment analysis showed that Gua Lou Xie Bai Gui Zhi Tang might participate in coronary heart disease treatment through Hypoxia-inducible factor-1 (HIF-1), Tumor necrosis factor (TNF), PhosphoInositide-3 Kinase–Threonine protein kinase (PI3K-Akt), and the calcium signal pathway.
Conclusions: This study reveals that the Gua Lou Xie Bai Gui Zhi Tang mechanism in coronary heart disease treatment has the characteristics of multi-components, multi-targets, and multi-pathways, which provides a theoretical basis for its clinical application and subsequent experimental verification.