Controlled ovarian hyperstimulation impairs the endometrium receptivity during the implantation window, resulting in a lower clinical pregnancy rate and a higher abortion rate. Our study explored the effect of electroacupuncture on the endometrial receptivity of COH rats.
Female rats were randomly divided into normal treatment (Normal), model treatment (Model), low-frequency electroacupuncture treatment and high-frequency electroacupuncture treatment. Rats in the Model, low-frequency electroacupuncture treatment, and high-frequency electroacupuncture treatment treatment groups were injected with pregnant mare serum gonadotropin and human chorionic gonadotropin (HCG) to establish a model of controlled ovarian hyperstimulation rats.
Compared with the Normal, the endometrial thickness, the number of pinopodes and amount of blastocyst implantation in the Model group were significantly reduced. Among them, the endometrial thickness and the amount of blastocyst implantation in the Model group were substantially decreased than those in the high-frequency electroacupuncture treatment group.
High-frequency electroacupuncture treatment could markedly reduce the protein expression levels of E-cadherin, β-catenin, and claudin-1 (CLDN1). During high-frequency electroacupuncture treatment treatment, the LIF/STAT3 signaling pathway of controlled ovarian hyperstimulation rats was enhanced.
In conclusion, electroacupuncture could improve the endometrium receptivity and promote the blastocyst implantation in controlled ovarian hyperstimulation rats by reducing cell adhesion molecules and enhancing the LIF/STAT3 signaling pathway.